Portal de investigación
Transcriptomic profile of epileptic children treated with ketogenic therapies.
Fecha de publicación:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Ruiz-Herrero, Jana
- Canedo-Villarroya, Elvira
- Correas, Angela G
- Pedron-Giner, Consuelo
Grupos y Plataformas de I+D+i
Abstract
Background: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed light on its possible mechanisms. Methods: Eight children with refractory epilepsy were enrolled in the study. Peripheral blood mononuclear cells were obtained before and after the children were treated with KDT for a minimum of 6 months. RNA was extracted and mRNA and miRNA profiling were performed and analyzed. Results: Our intervention with KDT significantly reduced the seizure number in seven of the eight paediatric patients treated and caused important changes in their gene expression profile. Our study reveals modifications in the transcription of 4630 genes and 230 miRNAs. We found that the genes involved in the protection against epileptic crises were among those mainly changed. These genes collectively encode for ion channels, neurotransmitter receptors, and synapse structural proteins. Conclusions: Together our results explain the possible mechanisms of KDT and reinforce its clinical importance in the treatment of epilepsy.
© 2022 The Author(s). Published by IMR Press.
Datos de la publicación
- ISSN/ISSNe:
- 0219-6352, 1757-448X
- Tipo:
- Article
- Páginas:
- 31-31
- PubMed:
- 35164467
Journal of Integrative Neuroscience IOS Press
Citas Recibidas en Web of Science: 7
Documentos
Filiaciones
Keywords
- Anticonvulsant; Epilepsy; Ketogenic diet; Ketogenic dietary therapies; Synapsis; Transcriptome; miRNome
Financiación
Proyectos y Estudios Clínicos
A multidisciplinary project to advance in basic mechanisms, diagnosis, prediction, and prevention of cardiac damage in reperfused acute myocardial infarction.
Investigador Principal: VICENT BODÍ PERIS
PIE15/00013 . INSTITUTO SALUD CARLOS III . 2016
INCORPORACIÓN DE NUEVAS ÁREAS TEMÁTICAS Y NUEVOS GRUPOS AL CONSORCIO CIBER
Investigador Principal: JOSE VIÑA RIBES
CB16/10/00435 . INSTITUTO SALUD CARLOS III
Cita
Ruiz J,Olaso G,Serna E,Canedo E,Correas AG,Gambini J,Gomez MC,Pedron C,Vina J. Transcriptomic profile of epileptic children treated with ketogenic therapies. J. Integr. Neurosci. 2022. 21. (1):p. 31-31. IF:1,800. (4).
Transcriptomic profile of epileptic children treated with ketogenic therapies. Ruiz J, Olaso G, Serna E, Canedo E, Correas AG, Gambini J, Gomez MC et al. Journal of Integrative Neuroscience. 2022 enero 28. 21 (1):31-31. DOI:10.31083/j.jin2101031. PMID:35164467.
