Portal de investigación
Estrogen Replacement Therapy Induces Antioxidant and Longevity-Related Genes in Women after Medically Induced Menopause.
Fecha de publicación:
Fecha Ahead of Print:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Ferrando, M
- Lopez-Grueso, R
- Edo, R
- Pellicer, A
Grupos y Plataformas de I+D+i
Abstract
Females live longer than males in many species, including humans, and estrogens are in part responsible for this protection against aging. We reported previously that estrogens can protect rats against oxidative stress, by inducing antioxidant and longevity-related genes. Thus, this study was aimed at confirming the ability of estrogens to upregulate antioxidant and longevity-related genes in humans. For this purpose, we selected 16 women of reproductive age (18-42 years old) undergoing a fertility treatment that includes a medically induced menopause, at the Valencian Infertility Institute. We took blood samples at each time point of the treatment (basal, induced menopause, estrogen, and estrogen plus progesterone replacement therapy). mRNA expression of antioxidant and longevity-related genes in peripheral blood mononuclear cells (PBMC) was determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Determination of reduced glutathione (GSH) in total blood was carried out using high-performance liquid chromatography (HPLC). As expected, we found that medically induced menopause significantly decreased sexual hormone (estrogens and progesterone) levels. It also lowered glutathione peroxidase (GPx), 16S rRNA, P21, and TERF2 mRNA expression and blood GSH levels. Estrogen replacement therapy significantly restored estrogen levels and induced mRNA expression of manganese superoxide dismutase (MnSOD), GPx, 16S rRNA, P53, P21, and TERF2 and restored blood GSH levels. Progesterone replacement therapy induced a significant increase in MnSOD, P53, sestrin 2 (SENS2), and TERF2 mRNA expression when compared to basal conditions. These findings provide evidence for estrogen beneficial effects in upregulating antioxidant and longevity-related genes in women.
Copyright © 2021 C. Borrás et al.
Datos de la publicación
- ISSN/ISSNe:
- 1942-0900, 1942-0994
- Tipo:
- Article
- Páginas:
- 8101615-8101615
- DOI:
- 10.1155/2021/8101615
- PubMed:
- 34539974
Oxidative Medicine and Cellular Longevity HINDAWI LTD
Citas Recibidas en Web of Science: 27
Documentos
Filiaciones
Financiación
Proyectos y Estudios Clínicos
INCORPORACIÓN DE NUEVAS ÁREAS TEMÁTICAS Y NUEVOS GRUPOS AL CONSORCIO CIBER
Investigador Principal: JOSE VIÑA RIBES
CB16/10/00435 . INSTITUTO SALUD CARLOS III
Scaling-up of and evidence-based intervention programme in older people with Diabetes and Frailty in LatinAmericam. DIABFRAIL-LATAM: DR. JOSÉ VIÑA 2018
Investigador Principal: JOSE VIÑA RIBES
825546 . EUROPEAN COMMISSION . 2019
Cita
Borras C,Ferrando M,Ingles M,Gambini J,Lopez R,Edo R,Mas C,Pellicer A,Vina J. Estrogen Replacement Therapy Induces Antioxidant and Longevity-Related Genes in Women after Medically Induced Menopause. Oxid Med Cell Longev. 2021. 2021. p. 8101615-8101615. IF:7,310. (2).
Estrogen Replacement Therapy Induces Antioxidant and Longevity-Related Genes in Women after Medically Induced Menopause. Borras C, Ferrando M, Ingles M, Gambini J, Lopez R, Edo R, Mas C et al. Oxidative Medicine and Cellular Longevity. 2021 septiembre 09. 20218101615-8101615. DOI:10.1155/2021/8101615. PMID:34539974.
