Portal de investigación
Srebf2 Locus Overexpression Reduces Body Weight, Total Cholesterol and Glucose Levels in Mice Fed with Two Different Diets.
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Autores de INCLIVA
Grupos y Plataformas de I+D+i
Abstract
Macronutrients represent risk factors for hyperlipidemia or diabetes. Lipid alterations and type 2 diabetes mellitus are global health problems. Overexpression of sterol regulatory element-binding factor (Srebf2) in transgenic animals is linked to elevated cholesterol levels and diabetes development. We investigated the impact of increased Srebf2 locus expression and the effects of control and high-fat, high-sucrose (HFHS) diets on body weight, glucose and lipid metabolisms in transgenic mice (S-mice). Wild type (WT) and S-mice were fed with both diets for 16 weeks. Plasma glucose, insulin and lipids were assessed (n = 25). Immunostainings were performed in liver, pancreas and fat (N = 10). Expression of Ldlr and Hmgcr in liver was performed by RT-PCR (N = 8). Control diet: S-mice showed reduced weight, insulin, total and HDL cholesterol and triglycerides (TG). HFHS diet widened differences in weight, total and HDL cholesterol, insulin and HOMA index but increased TG in S-mice. In S-mice, adipocyte size was lower while HFHS diet produced lower increase, pancreatic beta-cell mass was lower with both diets and Srebf2, Ldlr and Hmgcr mRNA levels were higher while HFHS diet produced a rise in Srebf2 and Hmgcr levels. Srebf2 complete gene overexpression seems to have beneficial effects on metabolic parameters and to protect against HFHS diet effects.
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Datos de la publicación
- ISSN/ISSNe:
- 2072-6643, 2072-6643
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/nu12103130
- PubMed:
- 33066385
Nutrients MDPI AG
Citas Recibidas en Web of Science: 3
Documentos
Filiaciones
Keywords
- cholesterol; atherosclerosis; lipoproteins; sterol regulatory element-binding protein 2 (SREBP-2); carbohydrate metabolism; lipid metabolism; high-fat; high-sucrose diet; transgenic mice
Financiación
Proyectos y Estudios Clínicos
Identificación de variantes genéticas protectoras frente al desarrollo de diabetes tipo 2 en octogenarios.
Investigador Principal: FELIPE JAVIER CHAVES MARTÍNEZ
PI17/00544 . INSTITUTO SALUD CARLOS III . 2018
Cita
Andres I,Blesa S,Vinue A,Gonzalez H,Real JT,Martinez S,Carretero J,Ferrandez A,Chaves FJ,Garcia A. Srebf2 Locus Overexpression Reduces Body Weight, Total Cholesterol and Glucose Levels in Mice Fed with Two Different Diets. Nutrients. 2020. 12. (10):3130. IF:5,717. (1).
Srebf2 Locus Overexpression Reduces Body Weight, Total Cholesterol and Glucose Levels in Mice Fed with Two Different Diets. Andres I, Blesa S, Vinue A, Gonzalez H, Real JT, Martinez S, Carretero J et al. Nutrients. 2020 octubre 14. 12 (10):DOI:10.3390/nu12103130. PMID:33066385.
