Portal de investigación
Plasma Extracellular Vesicles Play a Role in Immune System Modulation in Minimal Hepatic Encephalopathy.
Fecha de publicación:
Fecha Ahead of Print:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Durban, L
- Rubio, T
- Felipo, V
Grupos y Plataformas de I+D+i
Abstract
Minimal hepatic encephalopathy (MHE) is associated with changes in the immune system including an increased pro-inflammatory environment and altered differentiation of CD4+ T lymphocytes. The mechanisms remain unknown. Changes in extracellular vesicle (EV) cargo including proteins and miRNAs could play a main role as mediators of immune system changes associated with MHE. The aim was to assess whether plasma EVs from MHE patients played a role in inducing the pro-inflammatory environment and altered differentiation of CD4+ T lymphocyte subtypes in MHE patients. We characterized the miRNA and protein cargo of plasma EVs from 50 cirrhotic patients (27 without and 23 with MHE) and 24 controls. CD4+ T cells from the controls were cultured with plasma EVs from the three groups of study, and the cytokine release and differentiation to CD4+ T-cell subtypes were assessed. Plasma EVs from MHE patients had altered miRNA and protein contents, and were enriched in inflammatory factors compared to the controls and patients without MHE. EVs from MHE patients modulated the expression of pro-inflammatory IL-17, IL-21, and TNF-a and anti-inflammatory TGF-ß in cultured CD4+ T lymphocytes, and increased the proportion of Th follicular and Treg cells and the activation of Th17 cells. In conclusion, plasma EVs could play an important role in the induction of immune changes observed in MHE.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1422-0067
- Tipo:
- Article
- Páginas:
- -
- PubMed:
- 36293192
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 8
Documentos
Filiaciones
Keywords
- CD4+ T lymphocytes; extracellular vesicles; miRNAs; minimal hepatic encephalopathy
Financiación
Proyectos y Estudios Clínicos
Caracterización de las alteraciones neurológicas en pacientes con encefalopatía hepática mínima. Estudio de mecanismos implicados.
Investigador Principal: JUAN JOSÉ GALLEGO ROIG
ACIF/2018/284 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO . 2018
Caracterización de las alteraciones neurológicas y cerebrales en pacientes con encefalopatía hepática mínima. Contribucu¡ión de la Inflamación. Implicaciones diagnósticas y terapéuticas
Investigador Principal: MARÍA DEL CARMEN MONTOLIU FÉLIX
PI18/00150 . INSTITUTO SALUD CARLOS III . 2019
Caracterización de las alteraciones neurológicas y cerebrales en pacientes con encefalopatía hepática mínima. contribución de la inflamación. Implicaciones diagnósticas y terapéuticas.
Investigador Principal: MARÍA DEL CARMEN MONTOLIU FÉLIX
GRISOLIAP/2019/003 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO . 2019
ESTUDIO DE LAS ALTERACIONES NEUROLÓGICAS, CEREBRALES Y BIOQUÍMICAS EN PACIENTES CON ENFERMEDAD GRASA DEL HÍGADO. MECANISMOS MOLECULARES Y CELULARES.
Investigador Principal: FRANC CASANOVA FERRER
ACIF/2019/232 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO
CONTRATOS RIO HORTEGA 2019
Investigador Principal: MARIA PILAR BALLESTER FERRE
CM19/00011 . INSTITUTO SALUD CARLOS III . 2020
Cita
Gallego JJ,Fiorillo A,Casanova F,Urios A,Ballester MP,Durban L,Megias J,Rubio T,Cabrera A,Escudero D,Felipo V,Montoliu C. Plasma Extracellular Vesicles Play a Role in Immune System Modulation in Minimal Hepatic Encephalopathy. Int. J. Mol. Sci. 2022. 23. (20):12335. IF:5,600. (1).
Plasma Extracellular Vesicles Play a Role in Immune System Modulation in Minimal Hepatic Encephalopathy. Gallego JJ, Fiorillo A, Casanova F, Urios A, Ballester MP, Durban L, Megias J et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022 octubre 15. 23 (20):DOI:10.3390/ijms232012335. PMID:36293192.
