Portal de investigación
Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database.
Fecha de publicación:
Fecha Ahead of Print:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Ortega, Maria
- Gavara, Jose
- Perez-Sole, Nerea
Grupos y Plataformas de I+D+i
Abstract
Extracellular matrix (ECM) changes after myocardial infarction (MI) need precise regulation, and next-generation sequencing technologies provide omics data that can be used in this context. We performed a meta-analysis using RNA-sequencing transcriptomic datasets to identify genes involved in post-MI ECM turnover. Eight studies available in Gene Expression Omnibus were selected following the inclusion criteria. We compare RNA-sequencing data from 92 mice submitted to permanent coronary ligation or sham, identifying differentially expressed genes (p-value < 0.05 and Log2FoldChange = 2). Functional enrichment analysis was performed based on Gene Ontology biological processes (BPs). BPs implicated in response to extracellular stimulus, regulation of ECM organization, and ECM disassembly were detected soon after ischemia onset. ECM disassembly occurred between days one to seven post-MI, compared with ECM assembly from day seven onwards. We identified altered mRNA expression of 19 matrix metalloproteinases and four tissue inhibitors of metalloproteinases at post-infarcted ECM remodeling and altered transcriptomic expression of 42 genes encoding 26 collagen subunits at the fibrotic stage. To our knowledge, this is the first meta-analysis using RNA-sequencing datasets to evaluate post-infarcted cardiac interstitium healing, revealing previously unknown mechanisms and molecules actively implicated in ECM remodeling post-MI, which warrant further validation.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1422-0067
- Tipo:
- Article
- Páginas:
- -
- PubMed:
- 36555255
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 5
Documentos
Filiaciones
Keywords
- RNA-sequencing; extracellular matrix; meta-analysis; myocardial infarction
Financiación
Proyectos y Estudios Clínicos
INCORPORACIÓN DE NUEVAS ÁREAS TEMÁTICAS Y NUEVOS GRUPOS AL CONSORCIO CIBER
Investigador Principal: VICENT BODÍ PERIS
CB16/11/00486 . INSTITUTO SALUD CARLOS III
Resolution of microvascular obstruction after myocardial infarction. A multidisciplinary approach to assess the structural and clinical consequences and to evaluate new therapeutic options.
Investigador Principal: VICENT BODÍ PERIS
PI20/00637 . INSTITUTO SALUD CARLOS III . 2021
A multidisciplinary study to advance in the understanding of the basic mechanisms and clinical implications of microvascular obstruction after acute myocardial infarction. Exploration of novel diagnostic and therapeutic opportunities.
Investigador Principal: VICENT BODÍ PERIS
PROMETEO/2021/008 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO . 2021
Cita
Ortega M,Rios C,Gavara J,de Dios E,Perez N,Marcos V,Ferrandez A,Bodi V,Ruiz A. Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database. Int. J. Mol. Sci. 2022. 23. (24):15615. IF:5,600. (1).
Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database. Ortega M, Rios C, Gavara J, de Dios E, Perez N, Marcos V, Ferrandez A et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022 diciembre 09. 23 (24):DOI:10.3390/ijms232415615. PMID:36555255.
