Portal de investigación
Gene expression profile following an oral unsaturated fat load in abdominal obese subjects.
Autores de INCLIVA
Participantes ajenos a INCLIVA
- de Marco G
- Martínez-Valls JF
Grupos y Plataformas de I+D+i
Abstract
PurposeOur aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls.MethodsA total of 20 controls and 20 abdominal obese patients were studied. Metabolic parameters and oxidative stress markers were measured with standardized protocols. The whole gene expression at fasting state and after the OFLT (0, 4 and 8h) was analysed using human HT-12-v4 expression beadchips, from Illumina.ResultsWe found a significant decrease in plasma glucose, insulin and oxidative stress markers in abdominal obese patients and controls. We found beneficial metabolic postprandial gene expression in three genes: FKBP5, DDIT4 and DHRS9. Following an OFLT, the postprandial mRNA expression of FKBP5, and DDIT4 was downregulated while that of DHRS9 was overexpressed, both in nondiabetic normolipidemic subjects and in insulin-resistant subjects with abdominal obesity.ConclusionsOur results suggest that an OFLT rich in unsaturated fatty acids downregulates the expression of FKBP5, coding for the glucocorticoid receptor pathway, and that of DDIT4, involved in the oxidative stress response. These changes could favourably influence the insulin resistance and oxidative stress status in the postprandial state.
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature
Datos de la publicación
- ISSN/ISSNe:
- 1436-6207, 1436-6215
- Tipo:
- Article
- Páginas:
- 1331-1337
- PubMed:
- 29520627
EUROPEAN JOURNAL OF NUTRITION SPRINGER HEIDELBERG
Citas Recibidas en Web of Science: 4
Documentos
- No hay documentos
Filiaciones
Keywords
- Postprandial lipemia; Unsaturated fat; Abdominal obesity; Insulin resistance; Oxidative stress; Gene expression
Financiación
Proyectos y Estudios Clínicos
Estudio de nuevos mecanismos inflamatorios y angiogénicos asociados a la obesidad grave morbida: papel del eje CXCR3 y los receptores RORs.
Investigador Principal: LAURA PIQUERAS RUIZ
PI15/00082 . INSTITUTO SALUD CARLOS III . 2016
Cita
Garcia AB,Martinez S,Real JT,Marin P,de Marco G,Priego A,Martínez JF,Carmena R,Chaves FJ,Ascaso JF. Gene expression profile following an oral unsaturated fat load in abdominal obese subjects. Eur. J. Nutr. 2019. 58. (3):p. 1331-1337. IF:4,664. (1).
Gene expression profile following an oral unsaturated fat load in abdominal obese subjects. Garcia AB, Martinez S, Real JT, Marin P, de Marco G, Priego A, Martínez JF et al. EUROPEAN JOURNAL OF NUTRITION. 2019 abril 01. 58 (3):1331-1337. DOI:10.1007/s00394-018-1659-4. PMID:29520627.
