Portal de investigación
EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness.
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Autores de INCLIVA
Participantes ajenos a INCLIVA
- Rodriguez, F M
Grupos y Plataformas de I+D+i
Abstract
The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.
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Datos de la publicación
- ISSN/ISSNe:
- 2045-2322, 2045-2322
- Tipo:
- Article
- Páginas:
- 3723-3723
- PubMed:
- 32111877
Scientific Reports NATURE PUBLISHING GROUP
Citas Recibidas en Web of Science: 18
Documentos
Filiaciones
Financiación
Proyectos y Estudios Clínicos
Estudio traslacional sobre la hiperacetilación de proteínas como vía final de resistencia a fármacos anti-egfr en cánceres de alta incidencia clínica.
Investigador Principal: ANDRÉS CERVANTES RUIPEREZ
2012/140 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO . 2013
Enfermedad mínima residual en cánceres colorrectales de alto riesgo resecados. Valor de las biopsias líquidas en el seguimiento y análisis de la heterogeneidad tumoral.
Investigador Principal: ANDRÉS CERVANTES RUIPEREZ
PI15/02180 . INSTITUTO SALUD CARLOS III . 2016
Cita
Gimeno F,Riffo AL,Ayala G,Tarazona N,Gambardella V,Rodriguez FM,Huerta M,Martinez C,Monton J,Rosello S,Roda D,Cervantes A,Franco L,Lopez G,Castillo J. EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness. Sci Rep. 2020. 10. (1):p. 3723-3723. IF:4,379. (1).
EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness. Gimeno F, Riffo AL, Ayala G, Tarazona N, Gambardella V, Rodriguez FM, Huerta M et al. Scientific Reports. 2020 febrero 28. 10 (1):3723-3723. DOI:10.1038/s41598-020-60476-7. PMID:32111877.
