Portal de investigación
Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Fecha de publicación:
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Autores de INCLIVA
Participantes ajenos a INCLIVA
- Pérez-Moraga, R
- Castillo-Marco, N
- Ochando, A
- Ortega-Sanchís, S
- Parras, M
- Monfort-Ortiz, R
- Satorres-Perez, E
- Novillo, B
- Perales, A
- Gormley, M
- Roson, B
- Fisher, SJ
Grupos y Plataformas de I+D+i
Abstract
Endometrial decidualization resistance (DR) is implicated in various gynecological and obstetric conditions. Here, using a multi-omic strategy, we unraveled the cellular and molecular characteristics of DR in patients who have suffered severe preeclampsia (sPE). Morphological analysis unveiled significant glandular anatomical abnormalities, confirmed histologically and quantified by the digitization of hematoxylin and eosin-stained tissue sections. Single-cell RNA sequencing (scRNA-seq) of endometrial samples from patients with sPE (n = 11) and controls (n = 12) revealed sPE-associated shifts in cell composition, manifesting as a stromal mosaic state characterized by proliferative stromal cells (MMP11 and SFRP4) alongside IGFBP1+ decidualized cells, with concurrent epithelial mosaicism and a dearth of epithelial-stromal transition associated with decidualization. Cell-cell communication network mapping underscored aberrant crosstalk among specific cell types, implicating crucial pathways such as endoglin, WNT and SPP1. Spatial transcriptomics in a replication cohort validated DR-associated features. Laser capture microdissection/mass spectrometry in a second replication cohort corroborated several scRNA-seq findings, notably the absence of stromal to epithelial transition at a pathway level, indicating a disrupted response to steroid hormones, particularly estrogens. These insights shed light on potential molecular mechanisms underpinning DR pathogenesis in the context of sPE.
© 2025. The Author(s).
Datos de la publicación
- ISSN/ISSNe:
- 1078-8956, 1546-170X
- Tipo:
- Article
- Páginas:
- 502-513
- PubMed:
- 39775038
NATURE MEDICINE NATURE PORTFOLIO
Citas Recibidas en Web of Science: 25
Documentos
Filiaciones
Proyectos y Estudios Clínicos
Análisis transcriptómico a nivel de célula única y tejido endometrial completo del fallo en decidualización presente en pacientes con preeclampsia severa.
Investigador/a Principal TAMARA GARRIDO GOMEZ
PI19/01659 . INSTITUTO SALUD CARLOS III . 2020
HUMAN UTERUS CELL ATLAS
Investigador/a Principal CARLOS ANTONIO SIMÓN VALLÉS
874867 . EUROPEAN COMMISSION . 2020
AYUDAS PARA CONTRATOS PREDOCTORALES PARA LA FORMACIÓN DE DOCTORES 2019
Investigador/a Principal CARLOS ANTONIO SIMÓN VALLÉS
PRE2019-090770 . MINISTERIO DE CIENCIA E INNOVACIÓN . 2020
Cita
Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia. Muñoz I, Pérez R, Castillo N, Cordero T, Ochando A, Ortega S, Parras M et al. NATURE MEDICINE. 2025 febrero 01. 31 (2):502-513. DOI:10.1038/s41591-024-03407-7. PMID:39775038.
