Portal de investigación
Characterization of Vitronectin Effect in 3D Ewing Sarcoma Models: A Digital Microscopic Analysis of Two Cell Lines.
Fecha de publicación:
Fecha Ahead of Print:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Díaz-Martín, J
- Salguero-Aranda, C
- Acevedo-León, D
- de Alava, E
Grupos y Plataformas de I+D+i
Abstract
Ewing sarcoma (ES) is an aggressive bone and soft-tissue pediatric cancer. High vitronectin (VN) expression has been associated with poor prognosis in other cancers, and we aimed to determine the utility of this extracellular matrix glycoprotein as a biomarker of aggressiveness in ES. Silk fibroin plus gelatin-tyramine hydrogels (HGs) were fabricated with and without cross-linked VN and cultivated with A673 and PDX73 ES cell lines for two and three weeks. VN secretion to culture media was assessed using ELISA. Morphometric analysis was applied for phenotypic characterization. VN release to culture media was higher in 3D models than in monolayer cultures, and intracellular, intercellular, and pericluster presence was also observed. A673-HGs showed lower density of clusters but a proportion of larger clusters than PDX73-HGs, which presented low cluster circularity. The cluster density of A673-HGs without added VN was higher than with added VN and slightly lower in the case of PDX73-HGs. Furthermore, a culture time of three weeks provided no benefits in cluster growth compared to two weeks, especially in A673-HGs. These advances in 3D modeling and digital quantification pave the way for future studies in ES and other cancers to deepen understanding about intra- and intercellular heterogeneity and anti-adhesion VN therapies.
Datos de la publicación
- ISSN/ISSNe:
- 2072-6694, 2072-6694
- Tipo:
- Article
- Páginas:
- -
- PubMed:
- 39409975
Cancers MDPI
Documentos
- No hay documentos
Filiaciones
Keywords
- childhood cancer; digital quantification; extracellular matrix; hydrogels
Financiación
Proyectos y Estudios Clínicos
INCORPORACIÓN DE NUEVAS ÁREAS TEMÁTICAS Y NUEVOS GRUPOS AL CONSORCIO CIBER
Investigador Principal: ROSA NOGUERA SALVA
CB16/12/00484 . INSTITUTO SALUD CARLOS III
Neuroblastoma: estrategias mecanoterapéuticas basadas en modelos 3D y tumores derivados de pacientes con microambiente rico en vitronectina
Investigador Principal: ROSA NOGUERA SALVA
PI20/01107 . INSTITUTO SALUD CARLOS III . 2021
FPU20/05344 DR. ISAAC VIECO MARTÍ (2021/306)
Investigador Principal: ISAAC VIECO MARTÍ
FPU20/05344 . MINISTERIO EDUCACION, CULTURA Y DEPORTE . 2021
Cita
López A,Parra K,Vieco I,Granados S,Díaz J,Salguero C,Acevedo D,de Alava E,Navarro S,Noguera R. Characterization of Vitronectin Effect in 3D Ewing Sarcoma Models: A Digital Microscopic Analysis of Two Cell Lines. Cancers. 2024. 16. (19):3347. IF:4,400. (2).
Characterization of Vitronectin Effect in 3D Ewing Sarcoma Models: A Digital Microscopic Analysis of Two Cell Lines. López A, Parra K, Vieco I, Granados S, Díaz J, Salguero C, Acevedo D et al. Cancers. 2024 septiembre 30. 16 (19):DOI:10.3390/cancers16193347. PMID:39409975.
