Portal de investigación
Novel Fibrillar and Non-Fibrillar Collagens Involved in Fibrotic Scar Formation after Myocardial Infarction
Fecha de publicación:
Fecha Ahead of Print:
Autores de INCLIVA
Participantes ajenos a INCLIVA
- Ortega, M
- Fábrega-García, MM
- Gavara, J
- Padilla-Esquivel, JJ
- Martinez-Dolz, L
- Jimenez-Navarro, M
Grupos y Plataformas de I+D+i
Abstract
Following myocardial infarction (MI), adverse remodeling depends on the proper formation of fibrotic scars, composed of type I and III collagen. Our objective was to pinpoint the participation of previously unreported collagens in post-infarction cardiac fibrosis. Gene (qRT-PCR) and protein (immunohistochemistry followed by morphometric analysis) expression of fibrillar (types II and XI) and non-fibrillar (types VIII and XII) collagens were determined in RNA-sequencing data from 92 mice undergoing myocardial ischemia; mice submitted to permanent (non-reperfused MI, n = 8) or transient (reperfused MI, n = 8) coronary occlusion; and eight autopsies from chronic MI patients. In the RNA-sequencing analysis of mice undergoing myocardial ischemia, increased transcriptomic expression of collagen types II, VIII, XI, and XII was reported within the first week, a tendency that persisted 21 days afterwards. In reperfused and non-reperfused experimental MI models, their gene expression was heightened 21 days post-MI induction and positively correlated with infarct size. In chronic MI patients, immunohistochemistry analysis demonstrated their presence in fibrotic scars. Functional analysis indicated that these subunits probably confer tensile strength and ensure the cohesion of interstitial components. Our data reveal that novel collagens are present in the infarcted myocardium. These data could lay the groundwork for unraveling post-MI fibrotic scar composition, which could ultimately influence patient survivorship.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1422-0067
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/ijms25126625
- PubMed:
- 38928330
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 2
Documentos
Filiaciones
Keywords
- myocardial infarction; fibrosis; collagen
Financiación
Proyectos y Estudios Clínicos
Resolution of microvascular obstruction after myocardial infarction. A multidisciplinary approach to assess the structural and clinical consequences and to evaluate new therapeutic options.
Investigador Principal: VICENT BODÍ PERIS
PI20/00637 . INSTITUTO SALUD CARLOS III . 2021
A multidisciplinary study to advance in the understanding of the basic mechanisms and clinical implications of microvascular obstruction after acute myocardial infarction. Exploration of novel diagnostic and therapeutic opportunities.
Investigador Principal: VICENT BODÍ PERIS
PROMETEO/2021/008 . CONSELLERIA EDUCACION/INNOVACION,UNIVERSIDADES, CIENCIA Y SOCIEDAD DIGITAL/EMPLEO . 2021
Obstrucción microvascular tras un infarto de miocardio. Avances hacia una cuantificación automatizada, biomarcadores basados en técnicas ómicas y una estratificación de riesgo a largo plazo personalizada.
Investigador Principal: VICENT BODÍ PERIS
PI23/01150 . INSTITUTO SALUD CARLOS III . 2024
Cita
Novel Fibrillar and Non-Fibrillar Collagens Involved in Fibrotic Scar Formation after Myocardial Infarction. Ortega M, Fábrega MM, Molina T, Gavara J, de Dios E, Pérez N, Marcos V et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2024 junio 01. 25 (12):DOI:10.3390/ijms25126625. PMID:38928330.
