Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis

Fecha de publicación: 01/11/2023 Fecha Ahead of Print: 28/10/2023

Autores de INCLIVA

Elena Victoria De Dios Lluch

Autor
Victor Marcos Garcés

Autor
Francisco Javier Chorro Gascó

Autor
César Rios Navarro

Autor
Amparo Ruiz Sauri

Autor
Vicent Bodí Peris

Autor

Participantes ajenos a INCLIVA

Ortega, Maria
Molina-Garcia, Tamara
Gavara, Jose
Perez-Sole, Nerea

Grupos y Plataformas de I+D+i

Grupo de Investigación en Electrofisiología cardiaca experimental

Leading Researcher

Antonio M. Alberola Aguilar
Grupo de Investigación Traslacional en Cardiopatía Isquémica

Leading Researcher

Vicent Bodí Peris

Abstract

Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencing transcriptomic datasets. Using eight studies from the Gene Expression Omnibus, RNA-sequencing data from 92 mice submitted to different times of coronary ischemia or sham were chosen. Functional enrichment analysis was performed based on gene ontology biological processes (BPs). Apoptosis-related BPs are activated up to day 3 after ischemia onset, whereas endothelial proliferation occurs from day 3 onwards, including an overrepresentation of up to 37 genes. Endothelial apoptosis post-MI is triggered via both the extrinsic and intrinsic signaling pathways, as reflected by the overrepresentation of 13 and 2 specific genes, respectively. BPs implicated in new vessel formation are upregulated soon after ischemia onset, whilst the mechanisms aiming at angiogenesis repression can be detected at day 3. Overall, 51 pro-angiogenic and 29 anti-angiogenic factors displayed altered transcriptomic expression post-MI. This is the first study using RNA sequencing datasets to evaluate the genes participating in post-MI endothelium physiology and angiogenesis regulation. These novel data could lay the groundwork to advance understanding of the implication of ECs after MI.

Datos de la publicación

ISSN/ISSNe:: 1661-6596, 1422-0067
Tipo:: Article
Páginas:: -
DOI:: 10.3390/ijms242115698
PubMed:: 37958681

Documentos

Published Version CC BY 4.0

Métricas

Filiaciones

Ortega, Maria:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Molina-Garcia, Tamara:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Gavara, Jose:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
de Dios, Elena:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Perez-Sole, Nerea:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Marcos-Garces, Victor:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Chorro, Francisco J:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Rios-Navarro, Cesar:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Ruiz-Sauri, Amparo:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain
Bodi, Vicente:: Instituto de Investigación. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain

Instituciones Sanitarias. INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.; Centro de Biomateriales e Ingenieria Tisular, Universidad Politecnica de Valencia, 46010 Valencia, Spain.; Centro de Investigacion Biomedica en Red (CIBER)-CV, 28029 Madrid, Spain.; Cardiology Department, Hospital Clinico Universitario, 46010 Valencia, Spain.; Department of Medicine, University of Valencia, 46010 Valencia, Spain.; Department of Pathology, University of Valencia, 46010 Valencia, Spain