Biofluid Specificity of Long Non-Coding RNA Profile in Hypertension: Relevance of Exosomal Fraction.

Fecha de publicación: 06/05/2022 Fecha Ahead of Print: 06/05/2022

Autores de INCLIVA

Participantes ajenos a INCLIVA

Riffo-Campos, Angela L
Perez-Hernandez, Javier
Flores-Chova, Ana

Grupos y Plataformas de I+D+i

Grupo de Estudio de Riesgo Cardiometabólico y Renal

Established Researcher

Raquel Cortés Vergaz

Abstract

Non-coding RNA (ncRNA)-mediated targeting of various genes regulates the molecular mechanisms of the pathogenesis of hypertension (HTN). However, very few circulating long ncRNAs (lncRNAs) have been reported to be altered in essential HTN. The aim of our study was to identify a lncRNA profile in plasma and plasma exosomes associated with urinary albumin excretion in HTN by next-generation sequencing and to assess biological functions enriched in response to albuminuria using GO and KEGG analysis. Plasma exosomes showed higher diversity and fold change of lncRNAs than plasma, and low transcript overlapping was found between the two biofluids. Enrichment analysis identified different biological pathways regulated in plasma or exosome fraction, which were implicated in fatty acid metabolism, extracellular matrix, and mechanisms of sorting ncRNAs into exosomes, while plasma pathways were implicated in genome reorganization, interference with RNA polymerase, and as scaffolds for assembling transcriptional regulators. Our study found a biofluid specific lncRNA profile associated with albuminuria, with higher diversity in exosomal fraction, which identifies several potential targets that may be utilized to study mechanisms of albuminuria and cardiovascular damage.

Datos de la publicación

ISSN/ISSNe:: 1661-6596, 1422-0067
Tipo:: Article
Páginas:: -
DOI:: 10.3390/ijms23095199
PubMed:: 35563588

Documentos

Published Version CC BY 4.0

Métricas

Filiaciones

Perez-Hernandez, Javier:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain

Department of Nutrition and Health, Valencian International University (VIU), 46002 Valencia, Spain
Martinez-Arroyo, Olga:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Ortega, Ana:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Flores-Chova, Ana:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Redon, Josep:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain

CIBER. CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III, Minister of Health, 28029 Madrid, Spain

Instituciones Sanitarias. Internal Medicine Unit, Hospital Clinico Universitario, 46010 Valencia, Spain
Cortes, Raquel:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain