Decreased Urinary Levels of SIRT1 as Non-Invasive Biomarker of Early Renal Damage in Hypertension.

Fecha de publicación: 02/09/2020 Fecha Ahead of Print: 02/09/2020

Autores de INCLIVA

Grupos y Plataformas de I+D+i

Grupo de Estudio de Riesgo Cardiometabólico y Renal

Established Researcher

Raquel Cortés Vergaz
Grupo de Investigación sobre Riesgo Cardiometabólico y Diabetes

Leading Researcher

Sergio Martinez Hervas

Abstract

Sirtuins have become important players in renal damage in hypertension and diabetes, but their value as biomarkers is poorly assessed. The aims of the study were to evaluate the levels of sirtuin1 (SIRT1), and two miRNAs that regulate SIRT1 expression in hypertensive patients with incipient renal damage with and without diabetes. We quantified urinary SIRT1 and claudin 1 (CLDN1) mRNA and miR34-a and miR-200a levels by quantitative real-time polymerase chain reaction (RT-qPCR) from patients and in cultured podocytes treated with high glucose and angiotensin II. Western blot and fluorescence analyses were also performed. We found decreased SIRT1 levels in patients with increased urinary albumin excretion (UAE), the lowest with diabetes presence, and a strong association with UAE, discriminating incipient renal damage. In vitro experiments also showed SIRT1 overall decreases in podocyte cultures under treatment conditions. In urine samples, miR-34a was reduced and miR-200a increased, both related to UAE levels. However, both miRNAs were generally increased in podocyte cultures under high glucose and angiotensin-II treatment. These results show a significant urinary SIRT1 decrease in albuminuric hypertensive patients, strongly associated with albuminuria, suggesting that SIRT1 could be a potential and non-invasive method to assess incipient renal damage in hypertensive patients.

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)

Datos de la publicación

ISSN/ISSNe:: 1661-6596, 1422-0067
Tipo:: Article
Páginas:: -
DOI:: 10.3390/ijms21176390
PubMed:: 32887498

Documentos

Published Version CC BY 4.0

Métricas

Filiaciones

Martinez-Arroyo, Olga:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Ortega, Ana:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Galera, Miriam:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Solaz, Elena:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Martinez-Hervas, Sergio:: Instituciones Sanitarias. Service of Endocrinology and Nutrition, Hospital Clinico Universitario, 46010 Valencia, Spain
Redon, Josep:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Cortes, Raquel:: Instituto de Investigación. Cardiometabolic and Renal Risk Research Group, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain